Simultaneous determination of evodiamine and rutaecarpine of compound Wuzhuyu ointment by HPLC / 国际中医中药杂志
International Journal of Traditional Chinese Medicine
;
(6): 1101-1103, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-417449
ABSTRACT
Objective To establish a method for the determination of evodiamine and rutaecarpine of compound wuzhuyu ointment.Methods HPLC method was used with Agilent HC-C18(250 mm× 4.6 mm,5 μm) column and the mobile phase being acetonitrile-0.08% ocane sutfonic acid sodium(4159).The flow rate was 0.8 ml/min,the column temperature was 30℃,and the UV detector was set at 225 nm.Results The linear ranges of evodiamine and rutaecarpine were 0.62~ 12.40 μg/ml (r=0.9997) and 0.24~4.8 0 μg/ml (r=0.9996),respectively.The average recovery of evodiamine and rutaecarpine was 97.9% and 98.0%,respectively.Conclusion The method is simple,repeatable and accurate.It can be applied in quantitative determination of evodiamine and rutaecarpine in compound Wuzhuyu ointment.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
International Journal of Traditional Chinese Medicine
Year:
2011
Type:
Article
Similar
MEDLINE
...
LILACS
LIS