Effects of IL-8 on the tight junction of vascular endothelial cells / 国际生物医学工程杂志
International Journal of Biomedical Engineering
;
(6): 336-339, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-417554
ABSTRACT
Objective To investigate the influence of IL-8 on the tight junction of vascular endothelial cells.Methods Immunofluorescence was used to observe the modality and the distribution of three tight junction proteins (occludin,claudin-5 and ZO-1) of the EA.hy926 cells treated with IL-8 under different concentrations and different times.RT-PCR was used to measure the mRNA expression of these three proteins.Results The results demonstrated that IL-8 could change the distribution of occludin,claudin-5 and ZO-1 in EA.hy926 cells,and the mRNA expression of occludin,claudin-5 and ZO-1 decreased with the increase of IL-8 concentration and treated time.Conclusion The effects of IL-8 on the distribution and the expression of occludin,claudin-5 and ZO-1 are dose and time-dependent.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
International Journal of Biomedical Engineering
Year:
2011
Type:
Article
Similar
MEDLINE
...
LILACS
LIS