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Rapid Identification of Methylglyoxal Trapping Constituents from Onion Peels by Pre-column Incubation Method
Natural Product Sciences ; : 247-252, 2017.
Article in English | WPRIM | ID: wpr-41803
ABSTRACT
The methylglyoxal (MGO) trapping constituents from onion (Allium cepa L.) peels were investigated using pre-column incubation of MGO and crude extract followed by HPLC analysis. The peak areas of MGO trapping compounds decreased, and their chemical structures were identified by HPLC-ESI/MS. Among major constituents in outer scale of onion, 2-(3,4-dihydroxybenzoyl)-2,4,6-trihydroxy-3(2H)-benzofuranone (2) was more effective MGO scavenger than quercetin (6) and its 4′-glucoside, spiraeoside (3). After 1 h incubation, compound 2 trapped over 90% MGO at a concentration of 0.5 mM under physiological conditions, but compounds 3 and 6 scavenged 45%, 16% MGO, respectively. HPLC-ESI/MS showed that compound 2 trapped two molecules of MGO to form a di-MGO adduct and compounds 3 and 6 captured one molecule of MGO to form mono-MGO adducts, and the positions 6 and 8 of the A ring of flavonoids were major active sites for trapping MGO.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pyruvaldehyde / Quercetin / Flavonoids / Chromatography, High Pressure Liquid / Onions / Catalytic Domain / Methods Type of study: Diagnostic study Language: English Journal: Natural Product Sciences Year: 2017 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pyruvaldehyde / Quercetin / Flavonoids / Chromatography, High Pressure Liquid / Onions / Catalytic Domain / Methods Type of study: Diagnostic study Language: English Journal: Natural Product Sciences Year: 2017 Type: Article