Effects of Akt3 gene knockout on pain behaviors induced by chronic constriction injury of sciatic nerve in mice / 中华行为医学与脑科学杂志

ABSTRACT

ObjectiveTo investigate the effects of Akt3 gene knockout on neuropathic pain behaviors induced by chronic constriction injury of sciatic nerve (CCI).MethodsExperiment was divided into two groupsAkt3 knockout group (Akt3-/-,n =12),wild type group (WT,n =12 ).Randomly numbered,the right sciatic nerve of mice were received the operation of chronic constriction injury.Paw withdrawal mechanical threshold (PWMT)and paw withdrawal thermal latency (PWTL) were tested on day 1 before operation and day 1,3,5,7,10,14,17,21 afterCCI.ResultsThe basic values of PWMT(right(1.09±0.20)g,(1.17±0.22)g;left(1.17±0.15)g,(1.22±0.23)g,P＞0.05) andPWTL(right(6.18±1.11)s,(6.20±1.25)s;left(5.82±0.91)s,(5.92± 1.71 ) s,P ＞ 0.05 ) had no statistically significant differences between two groups.On day 1 after operation,compared with basic values,the PWMT and PWTL of the right paw in both Akt3-/- group and WT group decreased significantly (P ＜ 0.05 ),and at least lasted up to day 21.The PWMT( 3d(0.42 ± 0.22 ) g,(0.72 ± 0.36) g ; 17d(0.29 ±0.15)g,(0.49 ±0.19) g;21d(0.27 ±0.18)g,(0.56 ±0.15)g,P＜0.05) and PWTL(5d(2.43 ±0.68)s,(3.13±0.52)s;17d(2.43±1.26)s,(3.84±1.29)s ;21d(2.14±1.23)s,(4.07±1.26)s,P＜0.05 ) of the right paw in Akt3-/- group was significantly lower than those in WT group.The PWMT and PWTL of the left paw in Akt3-/- group and WT group had no obvious differences (P ＞ 0.05 ). However.compared to left paw,the PWMT and PWTL of the right paw of the two groups were obviously lower (P ＜ 0.05 ).ConclusionThe neuropathic pain induced by CCI increased in Akt3 gene knockout mice.