Effects of eicosapentaenoic acid on the growth of human cholangiocarcinoma cell lines FRH-0201 / 中华肝胆外科杂志

ABSTRACT

Objective To evaluate the effect of eicosapentaenoic acid (EPA) on the inhibition of proliferation and the induction of apoptosis of human cholangiocarcinoma cell lines FRH-0201.Methods Exponentially growing human cholangiocarcinoma cell lines FRH-0201 were exposed to EPA in vitro and cell growth was assessed by methylthiazolyl tetrazolium assay (MTT) assay,agarose gel electrophoresis and flow cytometry.Results After adding EPA,the proliferation of FRH-0201 cells were inhibited in a dose-dependent manner while apoptosis was induced.Flow cytometry detected apoptosis peaks and we found that superoxide dismutase (SOD) activity reduced significantly (P＜0.05,respectively; P＜0.001) while malondialdehyde (MDA) rose significantly (P ＜ 0.01,respectively; P＜0.001) after adding EPA with differing concentrations to human cholangiocarcinoma cell lines FRH-0201.Conclusions EPA can induce apoptosis of FRH-0201 cells in vitro and inhibit proliferation,possibly through increasing the lipid peroxidation.