Anti-inflammatory and Anti-oxidative Effects of Korean Red Ginseng Extract in Human Keratinocytes
Immune Network
;
: 42-49, 2011.
Article
in English
| WPRIM
| ID: wpr-41912
ABSTRACT
BACKGROUND:
In this study, we have investigated the effect of Korean red ginseng (KRG) extracts on the production of TNF-alpha and IL-8 in human keratinocytes. Also, to examine the antioxidative effect of red ginseng extracts, free radical scavenging activity and superoxide dismutase (SOD) activity in human dermal fibroblasts was measured.METHODS:
To investigate the effect of KRG in atopic dermatitis, we measured the level of TNF-alpha and IL-8 secretion in LPS-stimulated human keratinocytes after the treatment of KRG extracts using enzyme-linked immunosorbent assay. Anti-oxidative activity was investigated by measuring 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and SOD activity.RESULTS:
The stimulation of human keratinocytes with KRG extracts shifted the LPS-induced cytokine secretion toward a more immunosuppressive response. KRG dose-dependently decreased TNF-alpha and IL-8 production in HaCaT cells and a significant inhibition of TNF-alpha was shown when cells were treated with 500 and 1,000 microg/ml of KRG extracts. Additionally, KRG extracts showed DPPH radical scavenging and SOD activity in a dose-dependent manner. Particularly, SOD activities of concentrations higher than 60 microg/ml of KRG extracts were significantly different in human dermal fibroblast cells.CONCLUSION:
Based on this study, KRG extracts may be a useful immunosuppressive agent in the treatment of atopic dermatitis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Picrates
/
Superoxide Dismutase
/
Biphenyl Compounds
/
Enzyme-Linked Immunosorbent Assay
/
Keratinocytes
/
Interleukin-8
/
Tumor Necrosis Factor-alpha
/
Dermatitis, Atopic
/
Fibroblasts
/
Panax
Limits:
Humans
Language:
English
Journal:
Immune Network
Year:
2011
Type:
Article
Similar
MEDLINE
...
LILACS
LIS