Influence of MBL on the expression of Bcl-2 and p53 gene in thyroid cancer cell lines / 中国综合临床

ABSTRACT

Objective To observe the effect of recombinant human mannose-binding lectin (RhMBL)on the expression of Bcl-2 and p53 gene in thyroid cancer cell lines.Methods Two kinds of thyroid cancer cell lines( K1 cells and FTC-133 cells) were dealed with different concentrations of rhMBL( control group0 mg/L;experimental group1 mg/L),then total protein was extracted to detect the expression of Bcl-2 and p53 by Western Blot.Results The expression level of p53 and Bcl-2 were observed to be obviously down-regulated after incubation with RhMBL.( 1 ) K1 cellsThere was significant difference on the expression of p53 and Bcl-2 between control group and experimental group [ p53( 1.51 ± 0.29) vs (0.74 ±0.07),t =7.63 ; P =0.040;Bcl-2( 1.20 ± 0.07 ) vs ( 0.59 ± 0.04 ),t =6.28,P =0.001 ] ; ( 2 ) FTC-133 cellsthere was also significant difference on the expression of p53 and Bcl-2 between control group and experimental group [ p53( 0.88 ±0.14) vs(0.35 ±0.08),t =3.29,P=0.003; Bcl-2(1.07±0.11) vs (0.33 ±0.06),t =5.73,P=0.000 ].Conclusion Bcl-2 and p53 may play an important role on the induction of thyroid cancer cell apoptosis and inhibition its proliferation by RhMBL.