The influence on the proliferation of rat B lymphocytes induced by β1-adrenoceptor autoantibodies / 中华微生物学和免疫学杂志
Chinese Journal of Microbiology and Immunology
;
(12): 97-101, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-419522
ABSTRACT
Objective To investigate the influence of autoantibodies against the second extracellular loop of β1-adrenoceptor ( β1-AA) on the proliferation ability of LPS-stimulated rat B lymphocytes.Methods Active immunization assay was used to obtain adequate IgGs in which β1-AA was positive; MACS (magnetic activated cell sorting) assay was used for gaining rat splenic B lymphocytes; CCK8 assay was used for detecting the influence of β1-AA to the proliferation abilities of silent and LPS-stimulated rat splenic B lymphocytes.Results β1-AA (0.1 μmol/L pIgGs ) promoted the proliferation of LPS-stimulated rat splenic B lymphocytes(A values0.739±0.036 vs 0.533±0.032,P<0.05),and the effect was likely to be concentration-dependent.And the effect could be blocked by β1-AR blocker or β2-AR blocker partially,and could be blocked by β1-AR blocker and β2-AR blocker completely; β1-AA had no proliferation effect on silent rat splenic B lymphocytes.Conclusion β1-AA could promote the proliferation of LPS-stimulated rat splenic B lymphocytes via β1-AR and β2-AR which were on the surface of B lymphocytes.Thus,it could provide new clues for complex pathologic mechanism of cardiovascular patients in which β1-AA is positive.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Microbiology and Immunology
Year:
2012
Type:
Article
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