Growth inhibition and apoptosis of a multiple myeloma cell line induced by TLR3 pathway activation / 中华微生物学和免疫学杂志

ABSTRACT

Objective To investigate the roles of TLR3 pathway activiated by polyIC in proliferation and apoptosis of multiple myeloma (MM) RPMI8226 cell line.Methods RPMI8226 cells were cultured in RPMI 1640 with different dose of polylC.Cells were collected in different time.Proliferation and apoptosis were detected by CCK-8 kit and flow cytometry,separately.Results The proliferation of RPM18226 was inhibited by polyIC,and it was dose and time dependent,24 h12.30％ ±2.04％,22.50％±2.20％,37.90％ ±1.30％ ; 48 h17.80％ ±1.52％,29.60％ ±0.85％,45.80％ ±1.68％ ;72 h25.10％±1.01％,34.60％±1.27％,60.50％±2.08％,P＜0.05.RPMI8226 cells were incubated with 50 μg/ml,100 μg/ml and 200 μg/ml polyIC for 48 h.Apoptotic rate were 5.60％ ±1.06％,8.71％ ±1.06％ and 13.93％ ±1.17％,P＜0.05.TLR3 and TRIF mRNA expression increased obviously and dose dependent,TLR31.41±0.10,2.24±0.16,4.08±0.13; TRIF1.07±0.16,1.97±0.13,3.56±0.19,P＜0.05.Conclusion The proliferation of MM cells were inhibited by TLR3 pathway obviously,and apoptosis was induced by polyIC.