Acute toxicity and anti-stress effects of Naoxinshu capsule in mice / 国际中医中药杂志

ABSTRACT

Objective To investigate the acute toxicity and anti-stress effects of Naoxinshu capsule (NC) in mice.Methods In acute toxicity experiment,mice were oral administered to determine the maximum tolerance dose of NC.In anti-stress experiments,mice were divided into 5 groups at random,namely NC high,medium and low dose groups (2.4,1.2 and 0.6 g/kg,respectively),Gypenosides group(0.75 g/kg) and control group (n=10).After seven days of oral administration,rope climbing time,loaded swimming time,anti-hypoxia time,and survival rate under high or low temperature tests were carried out,respectively.Results The maximum tolerance dose of NC was 72 g/kg.NC high,medium and low dose groups significantly prolonged the rope climbing time [is (9.5 ± 3.1) min,(6.0± 1.6) min,(4.1 ± 1.2) min],loaded swimming time [is (28.2± 6.8) min,(25.7± 10.3)min,(22.9± 8.0)min] and anti-hypoxia time (around 17 min),compared to the control group (P＜0.05 or P＜0.01).NC high dose group significantly improved the survival rate of mice (i.e.30％) under high or low temperature (P＜0.05).Conclusion NC shows good anti-stress effects,and no detectable acute toxicity in mice.