An enzymatic method for the detection of human serum albumin
Experimental & Molecular Medicine
;
: 103-105, 2001.
Article
in English
| WPRIM
| ID: wpr-42074
ABSTRACT
Albumin is the most abundant protein in human serum. A dye-binding method is commonly used in clinical laboratories for its estimation using different types of dyes. However, all these dye methods were interfered by a variety of compounds. Here we present a method for the detection of albumin in human serum and other biological fluids. The principle is based on the fact that lactate dehydrogenase isoenzyme-5 (LDH-5) binds specifically to Dextran-Blue (DB). Albumin inhibits the binding of LDH-5 with DB. Absence of LDH activity in DB fraction after gel filtration indicates the presence of albumin in sample and vice versa.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Protein Binding
/
Sepharose
/
Serum Albumin
/
Chemistry, Clinical
/
Chromatography, Gel
/
Isoenzymes
/
L-Lactate Dehydrogenase
Type of study:
Diagnostic study
Limits:
Humans
Language:
English
Journal:
Experimental & Molecular Medicine
Year:
2001
Type:
Article
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