Clinical significance of peripheral blood PD-1 and its ligands in patients with anti-HBe positive CHB =or anti-HBs positive of normal ALT / 中华微生物学和免疫学杂志

ABSTRACT

Objective To study the clinical significance of peripheral blood PD-1 (CD279) and its ligands(CD274 and CD273)between patients with anti-HBe positive chronic hepatitis B (CHB) or anti-HBs positive group of normal glutamic acid alanine aminotransferase (ALT).Methods A total of 70 subjects of normal ALT,including 23 patients with anti-HBe positive,22 anti-HBs positive and 25 normal controls,were enrolled.The expression level of CD279,CD274 and CD273 in peripheral lymphocyte were detected by flow cytometry.Compare the group differences by multivariate analysis of variance.Results The expression level of CD279 in control group,anti-HBe positive group and anti-HBs positive group was (33.3±5.4)％,(48.0±9.1) ％,(42.8±9.0) ％,respectively.The expression level of CD274 in control group,anti-HBe positive group and anti-HBs positive group was (59.4 ±4.4) ％,(71.3± 10.3) ％ and (62.9 ± 10.2) ％,respectively.The expression level of CD273 in control group,anti-HBe positive group and anti-HBs positive group was (5.3±2.2)％,(15.6±5.3)％ and (5.1±1.0)％,respectively.The expression level of CD279 and CD274 decreased gradually from anti-HBe positive group to anti-HBs positive group and then to normal control group.Three dependent variables in the overall difference was statistically significant (Pillai trace =0.988,F=3090.105,P=0.000),η partial square was 0.458.The difference for CD273 expression level of anti-HBs positive group compared with control group was not statistically significant (P =0.082) ; for other groups pairwise comparisons were statistically significant(P＜0.01).Conclusion Monitoring the alteration of PD-1 and its ligands of the HBeAg seroconversion CHB patients and the anti-HBs positive population can better understand the changes in course of HBV infection,thereby improving the prognosis of patients.with HBV infection.