Evaluation of angiogenesis after stem cell transplantation using color-encoded parametric quantification with targeted microbubbles in rabbits with myocardial infarction / 中华超声影像学杂志
Chinese Journal of Ultrasonography
;
(12): 983-987, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-423305
ABSTRACT
Objective To investigate the application of parametric quantification (PQ) with targeted microbubbles on angiogenesis after stem cell transplantation in rabbits with myocardial infarction.Methods A CD34-targeted microbubble was prepared.Acute myocardial infarction (AMI) model were established by ligating coronary artery in 80 New Zealand white rabbits.Rabbits in common microbubble group (T + C group) and CD34-targeted microbubble group (T + T group) were subjected to myocardial contrast echocardiography(MCE),then compare myocardial perfusion parameters (A,β and A × β values) pre- and 3d post- ligation and 4 weeks after stem cell transplantation.Microvessel density (MVD) were determine by CD34 antibody immunohistochemistry.Results Compared to before stem cell transplantation,the perfusion scores ( P <0.05) and A,β and A× β values( P <0.01) of T+ C group and T+ T group were increased than before transplantation and control group.MVDs after stem cell transplantation in experimental groups were higher than those of control group ( P <0.01),especially the T + T group.A × β value in the T + C group was correlated with MVD ( r =0.658,P < 0.05) ; β and A × β value in the T + T group were correlated with MVD ( r =0.620,0.859,P <0.05).A × β value ( X ) and MVD ( Y ) were included to establish the following regression equationY =- 130.986 + 34.505 X ( R 2 =0.556,P < 0.05).Conclusions PQ and CD34-targeted microbubble probably provides some value in invasive evaluation of angiogenesis after stem cell transplantation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Prognostic study
Language:
Chinese
Journal:
Chinese Journal of Ultrasonography
Year:
2011
Type:
Article
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