Effect of Anti - idiotype Antibody on Anti - DNA Antibody Production by Hybridoma Cells / 대한면역학회지
Korean Journal of Immunology
;
: 513-524, 1997.
Article
in Korean
| WPRIM
| ID: wpr-42340
ABSTRACT
Anti-idiotype antibody (anti-id Ab) which recognizes idiotope in the variable region of immunoglobulin (Ig) can regulate Ab production by B cells in vivo and in vitro. Although it has been reported that anti-id Ab can suppress IgM production by lymphocytes or hybridoma cells without suppression of cell proliferation, the regulatory mechanism of anti-id Ab is not completely understood. We studied the effects of anti-id Ab on the production of IgG class anti-DNA Ab by hybridoma cells, on the proliferation of cells, and on the transcription levels of Ig genes. In contrast to suppressive effect of anti-id Ab on the production of IgM previously reported by others, stimulatory effects of anti-id Ab on the production of IgG by hybridoma cells as well as the proliferation of these .cells were observed. However, little effect of anti-id Ab on the transcription levels of Ig genes was observed. These results suggest that anti-id Ab can increase Ab production by stimulation of cell proliferation. Furthermore, these results suggest that the effect of anti-id Ab on the production of Ab may be determined by the difference in class of Ab produced by hybridoma cells following the treatment with anti-id Ab.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
DNA
/
Immunoglobulin G
/
Immunoglobulin M
/
Immunoglobulins
/
Genes, Immunoglobulin
/
B-Lymphocytes
/
Lymphocytes
/
Cell Proliferation
/
Hybridomas
/
Antibody Formation
Language:
Korean
Journal:
Korean Journal of Immunology
Year:
1997
Type:
Article
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