Diethylstilbestrol can induce bone marrow stromal cells to differentiate into osteoblasts / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
;
(53): 7429-7432, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-423829
ABSTRACT
BACKGROUND:
There are fewer reports about estrogen effects on bone marrow stromal cells (BMSCs).OBJECTIVE:
To study the effect of diethylstilbestrol on the osteogenic differentiation of rabbit BMSCs.METHODS:
Rabbit BMSCs cultured in vitro were intervened with 0, 10-7, 10-6, 10-5 mol/L diethylstilbestrol, and BMSCs cultured with dexamethasone 10-8 mol/L, β-sodium glycerophosphate 10 mmol/L, and vitamin C 50 mg/L were used as positive controls. RESULTS ANDCONCLUSION:
10-6 mol/L diethylstilbestrol significantly improved the proliferative ability of BMSCs at 24, 48, and 72 hours after intervention (P < 0.01). 10-5 mol/L diethylstilbestrol significantly inhibited the proliferation of BMSCs at 48 hours after intervention as well as 10-7 mol/L diethylstilbestrol at 72 hours (P < 0.01). Mineralized nodular structures formed at 25 days after intervention with 10-7 mol/L diethylstilbestrol. Alkaline phosphatase activities were remarkably increased at 14 and 21 days after intervention with 10-7, 10-6 mol/L diethylstilbestrol. It has been proved that diethylstilbestrol has an enhancing effect on the osteogenic differentiation of rabbit BMSCs.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Tissue Engineering Research
Year:
2011
Type:
Article
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