An in vitro study of rhBMP-2/ vancomycin/ calcium sulfate drug delivery system / 中华创伤骨科杂志
Chinese Journal of Orthopaedic Trauma
;
(12): 53-57, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-424482
ABSTRACT
Objective To study the efficacy and safety of in vitro local drug delivery capsules fabricated with the biodegradable material Osteoset(R)plus vancomycin and recombinant human bone morphogenetic protein-2 (rhBMP-2). Methods In vitro local drug delivery capsules were fabricated with the biodegradable material Osteoset(R) plus vancomycin and rhBMP-2.High-Performance Liquid Chromatography (HPLC) and bacterial inhibition tests were performed to detect the concentration and activity of vancomycin in the capsules.Enzyme-linked immunosorbent assay(ELISA) and alkaline phosphatase(ALP) tests were conducted to detect the concentration and activity of rhBMP-2 in the capsules. Results The capsules could release vancomycin above 55.8 μg/mL for up to 144 hours,with an activity of above 70%.They could release active thBMP-2 for up to 30 days,had no inhibition effects on the proliferation of mesenchymal stem cells(MSCs) and had high biological safety. Conclusion Since the capsules of Osteoset(R) plus vancomyein and rhBMP-2 can release high concentrations of active vancomycin and active rhBMP-2 with no inhibition of the proliferation of MSCs,they have good prospects for clinical use.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Orthopaedic Trauma
Year:
2012
Type:
Article
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