Effect of eicosapentaenoic acid or eicosapentaenoic acid plus carboplatin on proliferation and apoptosis of human lung cancer cell line A-549 / 中华临床营养杂志
Chinese Journal of Clinical Nutrition
;
(6): 30-32, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-424864
ABSTRACT
Objective To investigate the effects of eicosapentaenoic acid (EPA) or EPA plus carboplatin on the proliferation and apoptosis of human lung cancer cell line A-549.Methods A-549 cells were cultured by 100 μg/ml carboplatin,80 μg/ml EPA,and their combination for 48 hours.MTT assay was used to determine the effect of EPA,carboplatin,or their combination on the proliferation of human lung cancer cell line A-549.The morphological changes of human lung cancer cell line A-549 were observed using HE staining,and apoptosis of A-549 cells was analyzed by flow cytometry.Results MTT assay showed that the proliferation inhibition rate of A-549 cells cultured with EPA plus carboplatin was 85.20% ± 5.00%,which was significantly higher than that of cells cultured with 80 μg/ml EPA (32.85% ± 3.00%,P =0.0001 ) or 100 μg/ml carboplatin (53.25% ±3.00%,P =0.0013 ).HE staining showed apoptosis in all three groups,whereas the apoptosis rate reached 17.05% ± 4.00% in the combination group,which was significantly higher than that in carboplatin group (9.49% ± 1.00%,P =0.0252).Conclusion EPA may enhance the effects of carboplatin in inhibiting the proliferation and promoting the apoptosis of human lung cancer cell line A-549.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Clinical Nutrition
Year:
2012
Type:
Article
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