Expression and biological significance of mPGES-1 and COX-2 in lung cancer / 中国基层医药

ABSTRACT

ObjectiveTo explore the expression and biological significance of microsomal prostaglan din E synthase-1 ( mPGES-1 ) and cyclooxyenase-2 (COX-2) in lung cancer.MethodsThe expressions of mPGES-1 and COX-2 mRNA in cancerous tissue and tumor-adjacent tissue were detected by RT-PCR,while the immunohistochemical S-P method was used to evaluate the expression of mPGES-1 and COX-2 proteins in the corresponding tissues.ResultsThe mRNA level of COX-2 and mPGES-1 significantly increased( P ＜0.05) in lung cancer group as compared with those in the tumor-adjacent tissue.The positive expression rate of mPGES-1 in cancerous tissue and tumoradjacent tissue were 66.7％ (40/60) and 10.0％ (6/60) respectively.The positive expression rate of COX-2 protein in cancerous tissue and tumor-adjacent tissue were 73.3％ (44/60)and 13.3％ (8/60),respectively.The positive expression rate of COX-2 was significantly higher in lung cancer than m tumor-adjacent tissue( x =21.99,P ＜ 0.01 ).The positive expression rate of mPGES-1 and COX-2 protein in lung cancer was independent of size of tumor and histological type(P ＞0.05 ),but correlated with histological grade,lymph node metastasis and TNM staging.(P ＜0.05 ).ConclusionIt suggested that the expression of mPGES-1 and COX-2 in lung cancer may play an important role in the process of carcinogenesis,and may provide a clinical basis for the early diagnosis and targeted therapy of lung cancer.