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Study on the isoniazid induced cellular damage and the expressions of Fas/Fas ligand of HepG2 / 中华传染病杂志
Chinese Journal of Infectious Diseases ; (12): 402-406, 2012.
Article in Chinese | WPRIM | ID: wpr-427361
ABSTRACT
Objective To establish a model of isoniazid induced necrosis and apoptosis in HepG2 cell and to observe the expressions of Fas/Fas ligand (FasL) in this model.Methods HepG2 cells were treated with different dosages of isoniazid (0,1,2,4,6 and 8 mg/mL) or blank control for 24 hours.Flow cytometer was used to observe the cellular morphology of the HepG2 cell.Annexin V/propidium iodide double staining and flow cytometry were employed to detect the necrosis and apoptosis of HepG2 cells.The expressions of Fas/FasL on the cells were also determined by flow cytometry.The data were analyzed by one-way ANOVA.The comparisons between the drug groups and the control group were performed by using Dunnett t test. Results The higher the dose of isoniazid (4,6,8 mg/mL) was,the more necrosis and apoptosis were observed.In the 4,6 and 8mg/mL isoniazid arms,the total mortality rates were all higher than the control group [(32.1 ±7.5)%,(34.9±8.1)%,(38.2±9.4)% vs (7.2±1.5)% respectively](t=4.62,5.14 and 5.75,respectively; all P<0.01 ).The expression levels of Fas increased along with the dose of isoniazid increasing [(8.7±2.2)%,(11.5±2.8)%,(12.3±3.0)% and (10.6±2.9)% in isoniazid 2,4,6and 8 mg/mL arms,respectively],which were all higher than that in control arm [(3.1 ±0.8) %](t=2.97,P<0.05; t=4.46,P<0.01; t=4.88,P<0.01; t=3.98,P<0.05).Furthermore,the expressions of FasL increased as well when the dose of isoniazid increased.The expression levels of FasL were (16.2±3.5)%,(21.7±4.8)% and (18.7±4.9)%,respectively in isoniazid 4,6 and 8 mg/mL arms,which were all higher than that in the control group [(7.4±1.4)%](t=3.11,P<0.01; t=5.06,P<0.01; t=3.99,P<0.05).HepG2 cell necrosis increased with isoniazid of 8 mg/mL.However,the increase of apoptosis was not observed.Conclusion Isoniazid can induce HepG2 cell necrosis and apoptosis,and the apoptosis may be related with the increased expressions of the Fas/FasL on the cells.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Infectious Diseases Year: 2012 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Infectious Diseases Year: 2012 Type: Article