Performance Analysis of Panel Reactive Antibody Test by Lambda Antigen Tray Kit using Enzyme-Linked Immunosorbent Assay / 대한임상병리학회지
Korean Journal of Clinical Pathology
;
: 583-587, 2000.
Article
in Korean
| WPRIM
| ID: wpr-42783
ABSTRACT
BACKGROUND:
To detect human leukocyte antigen(HLA) alloantibodies, panel reactive antibody(PRA) test using complement dependent lymphocytotoxicity(CDC) has been generally used in Korea but this method lacks standardization. We analyzed the performance of Lambda Antigen Tray(LAT, One Lambda, Inc., Canoga Park, USA) kit using Enzyme-linked immunosorbent assay(ELISA).METHODS:
We carried out PRA screening by ELISA with LAT10X5 kit and CDC with home-made panels for 81 sera patients. We carried out PRA identification by ELISA with LAT1240 kit for some sera with positive or discordant results of both CDC and PRA screening by ELISA.RESULTS:
The agreement of CDC and PRA screening by ELISA was 85%(69/81). In 12 sera with positive results of both CDC and PRA screening by ELISA, no sera had same results of HLA antibody specificities. HLA class II antibodies were found in 13 sera(16%) and 12 sera of those had HLA class I antibodies, too.CONCLUSIONS:
The agreement of CDC and PRA screening by ELISA was similar to other foreign result. Disagreement of both methods might be due to greatly difference of frequencies of HLA in used panels. New manufactured goods considered frequencies of Korean HLA should be made in order that PRA by ELISA can be applied to routine work.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Complement System Proteins
/
Enzyme-Linked Immunosorbent Assay
/
Mass Screening
/
Isoantibodies
/
Korea
/
Leukocytes
/
Antibodies
/
Antibody Specificity
Type of study:
Screening study
Limits:
Humans
Country/Region as subject:
Asia
Language:
Korean
Journal:
Korean Journal of Clinical Pathology
Year:
2000
Type:
Article
Similar
MEDLINE
...
LILACS
LIS