Effect of NF-κB ODN decoy-treated DC on serum IFN-γ, IL-10 and anti Ⅱ -collagen antibody of collagen-induced arthritis rats / 中华微生物学和免疫学杂志

ABSTRACT

Objective To study NF-κB ODN decoy-treated DC on type Ⅱ collagen- induced arthritis(CIA) serum IFN-γ,IL-10 and anti B Ⅱ C antibody and its mechanism.Methods Type Ⅱ collagen induced arthritic rats was established.NF-κB decoy-treated DC loaded with B Ⅱ C from rat spleen were injected to CIA rats via the tail vein at 5 days after the initial immunization.The rats were divided into control group,CIA model group and B Ⅱ C-decoy-DC experimental groups.Forty-two days after the initial immunization,the arthritis scores were determined,the ankle pathology examination were performed and the levels of the serum IFN-γ,IL-10,anti-Ⅱ collagen antibody were determined using ELISA.Results The levels of IFN-γ and anti-B Ⅱ C antibody in serum of CIA model group were increased significantly and IL-10 was decreased significantly (P＜0.05,vs control group).After received with NF-κB ODN decoy inducing DC loaded with B Ⅱ C,the levels of IFN-γ and anti-B Ⅱ C antibody in serum of B Ⅱ C-decoy-DC experimental groups were decreased significantly and IL-10 was increased significantly (P＜0.05,vs CIA model group).Conclusion NF-κB decoy-treated DC loaded with B Ⅱ C had significantly inhibited IFN-γ and anti-B Ⅱ C antibody production and promoted increased levels of IL-10,and had a good curative effect on the treatment of rheumatoid arthritis.