Rat model of blood-ocular barrier breakdown induced by anterior segment intraocular analogic surgery / 中华眼底病杂志

ABSTRACT

Objective To establish a rat model of blood ocular barrier breakdown induced by anterior segment intraocular analogic surgery. Methods One hundred and fifty healthy adult male rats were randomly divided into control group and model group,75 rats in each group.The rats were anesthetized with 1 ml/kg ketamine hydrochloride/xylazine hydrochloride solution.Three way pipes were attached to a phosphate buffer infusion bag and two intravenous catheters. One catheter was inserted 30° obliquelythrough the transparent cornea anterior to the limbus into the rat's anterior chamber.Then the needle was withdrawn and the sheath was indwelling.Another catheter was connected with a manometer.Intraocular pressure was varied from 0 to 12 mm Hg (1 mm Hg=0.133 kPa) 60 times,30 times per min.The catheter was removed.The eyes were treated with ofloxacin ophthalmic solution after surgery.The 1st,2nd,3rd,5th and 7th day after surgery,the integrity of the blood ocular barrier was assessed by immunohistochemical staining for albumin and quantitative measurement using Evan's blue as a tracer. Results Albumin immunohistochemical staining of the control group was confined to the iris and retinal blood vessels.The choroid was stained at each time point after surgery.Albumin immunohistochemical staining of the model group was abundant around the iris and the retinal vasculature on the 1st day after surgery.The albumin diffused throughout the iris and the retina on the 2nd and the 3rd day after surgery.The albumin reached the retinal vessels on the 5th and 7th day after surgery.The aqueous humor Evans blue leakages of the model group were higher than those of the control group on the 1st,2nd,3rd and 5th day after surgery.The differences were statistically significant (t=25.781,37.433,25.150,19.171; P＜0.01).The Evans blue leakage of the model group was close to that of the control group on the 7th day after surgery. The difference was no statistical significant(t=1.303,P=0.209).The retinal Evans blue leakages of the model group were higher than those of the control group on the 1st,the 2nd and the 3rd day after surgery.The differences were statistically significant (t=11.997,14.622,23.014; P＜0.01).The Evans blue leakage of the model group was close to those of the control group on the 5th and 7th day after surgery. The differences were not statistically significant(t=2.027,0.756 ； P=0.058,0.459).Conclusion This study establishes a rat model of blood ocular barrier breakdown induced by imitating the injury to the anterior segment during intraocular surgery.