Polymorphism within the tumor necrosis factor receptor H ( TNFR Ⅱ ) in patients with ankylosing spondylitis in hunan province / 中华微生物学和免疫学杂志

ABSTRACT

Objective To investigate the association between ankylosing spondylitis (AS) in Han population from Hunan province of China and polymorphism located at the position nt587 in the tumor necrosis factor receptor Ⅱ ( TNFR Ⅱ ) gene.Methods Correlation analysis was performed in a case-control of 100 AS cases and 100 healthy control.The SNPs located in the TNFR Ⅱ gene ( TNFR Ⅱ nt587 ) was examined by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP).With the SPSS software,the relationship between AS and frequencies of genotype and alleles in TNFR Ⅱ nt587 were analyzed.Results There were 43 cases with TNFR Ⅱ nt587 T/T genotype,32 cases with TNFR Ⅱ nt587 T/G genotype and 25 cases with TNFR Ⅱ nt587 G/G genotype in the AS cases.While in the healthy control,there were 56 cases with TNFR Ⅱ nt587 T/T genotype,34 cases with TNFR Ⅱ nt587 T/G genotype and 10 cases with TNFR Ⅱ nt587 G/G genotype.At the same times,the allele frequencies of G in AS group was significantly higher ( x2 =8.734,P=0.003 ) than the control group (41.0％ vs.27.0％ ).The odds ratio in AS cases with TNFR Ⅱ nt587 G/G genotype was 3.256,which higher than those with G/G ( OR =1.226) genotype and T/T genotype compared the healthy control.Conclusion The study demonstrates that polymorphism within the TNFR Ⅱ at the position nt587 is associated with AS and TNFR Ⅱ nt587 G may play an important role in AS susceptibility,and TNFR Ⅱ nt587 G/G genotype may increase the sicken risk of AS in Hunan population.