Detection of beta-lactamase, porin-coding genes and linkage of KPC-ISKpn6 of the carbapenems-resistant Klebsiella pneumoniae / 中华微生物学和免疫学杂志
Chinese Journal of Microbiology and Immunology
;
(12): 953-958, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-429342
ABSTRACT
Objective To investigate the resistance-mechanism of the carbapenems-resistant Klebsiella pneumoniae isolated from clinical.Methods The clinical isolates of carbapenems-resistant Klebsiella pneumoniae from top three comprehensive hospitals of Nanjing area were examined by 40 beta-lactamase,porin-coding genes and linkage of KPC-ISKpn6 using PCR method,the PCR positive results were picked out for sequencing and sequencing BLAST search for comparison analysis.Results Twenty-four strains of carbapenems-resistant Klebsiella pneumoniae were detected,the positive rate of A beta-lactamase TEM-1 and SHV was 100% (24/24),KPC-2 and LAP-2 was 95.8% (23/24),45.8% (11/24) respectively,and C beta-lactamase DHA was 4.2% (1/24).Meanwhile,the positive detection rates of KPC-ISKpn6 linkage was 95.8% (23/24),and the mutation rate of porin-coding genes ompK35 and ompK36 were up to 95.8% (23/24) and 100% (24/24).Conclusion High incidence of beta-lactamase TEM-1,SHV,KPC-2 and LAP-2 was found in the group of Klebsiella pneumoniae isolates,and carbapenems-resistant of which was primarily due to the high carrying rate of KPC-2 and the high mutation rate of porin-coding genes ompK35 and ompK36.The Insertion sequence ISKpn6 may be involved in the KPC-2 gene mediated-expression.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
Language:
Chinese
Journal:
Chinese Journal of Microbiology and Immunology
Year:
2012
Type:
Article
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