Establishing the combined bacterial detection procedure for the diagnosis of bacterial vaginosis / 中华检验医学杂志

ABSTRACT

Objective To discuss the best bacterial combination for the diagnosis of Bacterial vaginosis (BV).Methods This is a retrospective study,230 BV-positive patients and 360 healthy women were enrolled based on the Amsel criteria and Nugent score.5 BV-associated bacteria,including Gardnerella vaginalis,Atopobium vaginae and Leptotrichia/Sneathia species,et al.were amplified by specific-PCR assay,the detection rate were compared between two groups.ROC curve and Kappa test were used to select the best combination.Results The detection rate of Gardnerella vaginalis,Atopobium vaginae,Leptotrichia/Sneathia species,Megasphaera species and Mobiluncus mulieris in BV group (91.3％,83.5％,39.1％,42.6％ and 36.5％ respectively) were markedly higher than that in healthy women (37.2％,14.4％,11.7％,8.9％ and 5.6％ respectively),x2 value were 168.848,275.776,60.949,92.886 and 92.68,all P ＜ 0.05.The area under ROC curve of A.vag,G.Vag + A.vag,G.Vag + A.vag + Lepto,G.Vag + A.vag +Mega and G.Vag + A.vag + M.mul were 0.845,0.862,0.865,0.869 and 0.867,and the sensitivity and specifity were higher than 80％,the value of Kappa were larger than 0.75 (P ＜ 0.05).Thus,these five methods were coincident.Conclusion Detection of Atopobium vaginae may be a better way for the diagnosis of BV.