Determination of gallic acid in chebulae fructus immaturus of different area by RP-HPLC / 国际中医中药杂志

ABSTRACT

Objective To establish an HPLC method for determining gallic acid in Chebulae fructus immaturus of different area.Methods The ZORBAX SB-C18 (250 mm×4.6 mm,5 μm) column was used,the mobile phase consisted of acetonitrile 0.1％ H3PO4(29 71),the flow rate was 1.0 ml/min,the column temperature was 30℃ the detecting wavelength was at 273 nm.Results Gallic acid was successfully separated within 20 min,the linear response range was 0.5611～5.611 μg.The average recovery was 99.9％,and RSD was 2.35％.Conclusion The method is simple,accurate and repeatable; it can provide evidence for further development and utilization of this crude drug.