In vitro effects of hyperbaric oxygen and DDP on proliferation of lung adenocarcinoma cell line A549/DDP / 中华胸心血管外科杂志
Chinese Journal of Thoracic and Cardiovascular Surgery
; (12): 617-619, 2012.
Article
in Zh
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| ID: wpr-430495
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ABSTRACT
Objective To evaluate the effects of hyperbaric oxygen (HBO) and cisplatin (DDP) on inhibition of A549/DDP cells in vitro and to explore the impacts of HBO on cell cycle and apoptosis.Methods The cytotoxicity of HBO and DDP was determined by Methyl thiazolyl tetrazolium (MTF) assay; flow cytometry (FCM) was used to investigate the impacts on cell cycle and apoptosis of cell line A549/DDP.Results Hyperbaric oxygen alone significantly suppressed A549/DDP cells proliferation (P < 0.05),compared with control group of normal oxygen,and the inhibition ratio was 2.90%.DDP at concentrations ranging from 3 μg/ml to 24 μg/ml significantly inhibited the proliferation of A549/DDP cells in a dose dependent manner,that was concentrations of 3,6,12 and 24 μg/ml brought inhibition ratio of 1.87%,4.62%,11.15% and 30.45% ; Hyperbaric oxygen can enhance the inhibition effect of DDP to A549/DDP cells,when hyperbaric oxygen affiliated with DDP of concentrations above,the inhibition ratios were 4.37%,8.92%,21.88% and 48.71%,respectively,which has significant difference with the single DDP groups.FCM analyses showed that HBO arrested A549/DDP cells at the S phase,that was cell number of G0/G1 phase reduced significantly and of S phase raised significantly,compared with normal oxygen group(P <0.01),that was the percentages of cells in S phase of control group were 9.5% and HBO group were 20.9%,while the percentages of cells in G0/G1phasc were 82.2% and 66.3%,respectively.In addition,there were more apoptotic cells in the HBO group with the percentage of 7.9%,while the percentage was 2.8% in the normal oxygen group.Conclusion Hyperbaric oxygen could play together with DDP to inhibit A549/DDP cells proliferation.HBO could block the cell cycle at S period and induce apoptosis.
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WPRIM
Language:
Zh
Journal:
Chinese Journal of Thoracic and Cardiovascular Surgery
Year:
2012
Type:
Article