The effect of Brassica rapa L-Polysaccharide on inflammatory response with asthmatic rats / 中国医师杂志

ABSTRACT

Objective To investigate the effect of Brassica rapa L-Polysaccharide on inflammatory response with asthmatic rats.Methods Forty Wistar rats were randomly divided into normal control group,model group,low-dose group and turnip large-dose group of Brassica rapa L.extract.The rats of model group,low-dose group and turnip large-dose group of Brassica rapa L-Polysaccharide were made into the model of asthma.The rats of low-dose group and turnip large-dose group of Brassica rapa L-Polysaccharide were given Brassica rapa L-Polysaccharide from gavage with 5 mg/kg and 2.5.mg/kg,and the rats of normal control group and model group were given distilled water,lasting two weeks.The serum inflammatory factor levels and bronchoalveolar lavage fluid (BALF) cell number of each group of asthmatic rats was detected.Results The serum levels of TNF-alpha,IL-6,IL-2 and CRP of low-dose and high-dose groups of asthmatic rats were significantly lower than the model control group[(90.33 ± 5.90)pg/ml,(92.01 ± 3.26)pg/ml vs (136.43 ± 7.81) pg/ml; (19.08 ± 1.27) pg/ml,(18.33 ± 2.09) pg/ml vs (25.14 ± 2.31) pg/ml;(115.36 ± 7.27) pg/ml,(110.72 ± 10.05) pg/ml vs (193.16 ± 12.33) pg/ml ; (70.58 ± 14.85) μg,/ml,(71.69 ± 12.11) μg/ml vs (93.21 ± 17.25) μg/ml],with a statistically significant difference (t =3.144～ 17.188,P ＜0.01) ; The total cells,eosinophils ratio and proportion of BALF lymphocytes of low-dose and high-dose groups of asthmatic rats were significantly lower than the control group[(1.58 ± 0.67) × 109/L,(1.55 ±0.89)×109/L vs (3.05 ±1.21) × 109/L;(20.31 ±4.27)％,(21.06±3.53)％ vs (26.35± 5.91) ％ ; (16.77 ± 5.47) ％,(15.89 ± 6.03) ％ vs (23.56 ± 4.81) ％],with a statistically significant difference(t =2.430 ～ 3.158,P ＜ 0.05,P ＜ 0.01).Conclusions The Brassica rapa L.Polysaccharide can effectively inhibit the secretion of various inflammatory factors in rats of asthma,and inflammatory response occurred.