Effect of Angiotensin II Receptor Inhibitor on Proliferation and Apoptosis of a Human Endometrial Cancer Cell Line Ishikawa Induced by Estrogen / 中国医科大学学报

ABSTRACT

Objective To explore the effect of Angiotensin Ⅱreceptor inhibitor on proliferation and apoptosis and cell cycle of human en-dometrial cancer cell line induced by estrogen.Methods Human endometrial cancer cells,ishikawa were cultured,and then treated with different concentrations of estrogen or saralasin.Cell proliferation was examined using MTT,and cell cycle and apoptosis were determined us-ing flow cytometry.Results Saralsin inhibited the proliferation of ishikawa cells.After treated with saralasin at 10 min after estrogen induc-tion,cycle was arrested at G1/G0 phase and S phase was reduced in ishikawa cell with significant difference between treatment group and control group.An increase in early apoptosis and late apoptosis occurred at 10min after treatment.Conclusion Saralsin inhibits endometrial cancer cell ishikawa proliferation,induces apoptosis and accumulation in G1/G0 phase in vitro.Therefore,saralsin may be helpful for the treat-ment of endometrial cancer.