Oxaliplatin Enhances TRAIL-induced Apoptosis in Gastric Cancer Cells by Inhibiting PI3K/Akt Pathway / 中国医科大学学报

ABSTRACT

Objective To investigate the role of phosphoinositide 3-kinases （PI3K）/Akt signaling pathway in TRAIL-induced cell apoptosis, and the effect of oxaliplatin on TRAIL-induced apoptosis in gastric cancer BGC823 cells. Methods Cell proliferation was roeasured using MTT assay. Cell apoptosis was determined by flow eytoroetry. The expression of Akt and phospbor-Akt were determined by Western blotting. Results 100 ng/mL TRAIL caused little cell apoptosis in BGC823 cells. TRAIL activated P13K/Akt pathway. Pretreated with PI3K in- hibitor LY294002 （25 μmol/L）for 1 h followed by exposure to TRAIL for 16 h,the cell apoptosis was obviously higher （12.7%±3,1% vs 3.5%±1.1% ,P 〈 0.05） than that without the treatment of LY294002. Treatment with 38 μg/mL oxaliplatin blocked the activation of P13K/ Akt signaling, and enhanced the sensitivity of cells to TRAIl,, the rate of cell apoptosis increased to 35.5%±4.5% （P 〈 0.05 ）. Conclusion Oxaliplatin enhanced the sensitivity of gastric cancer BGC823 cells to TRAIL by inhibiting TRAIL-induced the activation of PI3K/Akt pathway.