Effect of Jiawei Wendan decoction regulating CaMK/MAPK signal pathway in hippocampus of depression model rats / 中华行为医学与脑科学杂志
Chinese Journal of Behavioral Medicine and Brain Science
;
(12): 1064-1066, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-432858
ABSTRACT
Objective To explore the effect of Jiawei Wendan decoction on whole regulating function of CaMK/MAPK signal pathway in hippocampus of depression model rats.Methods Isolated depression model rats as research object were living unpredictable chronic stress.Based on the examination of mRNA/protein expression which was the key factor of the signal pathway of CaMK/MAPK and the intersection CREB1 mRNA expression,the relationship between CaMK Ⅱ/RSK protein expression and the CREB1 mRNA expression were analyzed with relation analysis method.Results ① Bidirectional correlation analysis:
the respective coefficient (r value) of CaMK Ⅱ protein expression and the CREB1mRNA expression in hippocampus of the model group,Chinese medicine treatment group and western medicine treatnent group were-0.502 (P < 0.01),-0.643 (P < 0.01),-0.408 (P< 0.05) ;the respective coefficient(r value) of RSK protein expression and the CREB1 mRNA expression in hippocampus of the model group,Chinese medicine treatment group and western medicine treatment group were 0.550 (P < 0.01),0.687 (P < 0.0 l),0.407 (P < 0.01).②Regressionanalysis:
the respective regression coefficient of CaMK Ⅱ protein (positive direction),RSK protein (negative direction) and CREB1 mRNA expression in the model group,Chinese nedicine treatment group and western medicine treatment group were R 2 =0.472,F =12.983(P<0.01),R2=0.666,F=28.961(P<0.01),R2=0.356,F=8.004(P<0.01).Conclusion The anti-depressant effect mechanism of Jiawei Wendan decoction is to regulate the whole function of the CaMK and MAPK signal pathway and then further up-regulate CREB1 mRNA expression.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Prognostic study
Language:
Chinese
Journal:
Chinese Journal of Behavioral Medicine and Brain Science
Year:
2012
Type:
Article
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