Effect of sanguinarine on growth and radiosensitivity of human glioma A172 cells / 中华放射医学与防护杂志

ABSTRACT

Objective To study the effect of sanguinarine on growth and radiosensitivity of human glioma A172 cells.Methods MTT assay was used to evaluate cell growth.Cell cycle analysis and reactive oxygen species(ROS) burst were performed by flow cytometry assay.Annexin V/PI assay was used to detect cell apoptosis.Colony formation assay was used to detect the influence of sanguinarine on cell radiosensitivity.Results Exposure of A172 cells to sanguinarine led to dose-and time-dependent cytotoxicity with IC50 values of 4.8,3.9 and 3.2 μmol/L for 12,24 and 48 h,respectively.Furthermore,sanguinarine caused an arrest of S phase.After being treated with 5 μmol/L sanguinarine for 24 h,the ratios of early apoptosis,late apoptosis and necrosis were (60.01 ± 3.73)％,(2.70 ± 0.18)％ and (3.93 ± 0.76)％ respectively in A172 cells compared with the untreated control(t =55.28,8.32,9.51,P ＜0.05).An increased generation of ROS was found after treatment with sanguinarine,however,NAC inhibited the effect of sanguinarine.As analyzed with multi-target click model fitting curves,the SERD0 of sanguinarine-treated cells was 1.48.Conclusions Sanguinarine inhibits the A172 cells growth via apoptosis induction and ROS burst.Moreover,sanguinarine at a non-cytotoxicity dose increases cell sensitivity to X-rays.