Determination of tetrahydropalmatine and rhynchophylline and isorhynchophylline in Anshen Yangxue Oral Liquid by HPLC / 中成药

ABSTRACT

AIM: To establish the method for determining tetrahydropalmatine,rhynchophylline and isorhynchophylline in AnshenYangxue Oral Liquid (Ramulus uncariae cum uncis, stephania kwangsiansis, Radix polygoni multiflori praeparata cum succo glycinus sotae , Caulis Polygoni multiflori and Pine needle). METHODS: Tetrahydropalmatine,rhynchophylline and isorhynchophylline were determined by HPLC. Chromatographic condition was composed of Kromasil C_(18) column, a mixture of methanol and water(55 45) as mobile phase with 0.01 mol/L triehthylamine, adjusted with acetic acid to pH of 7.5, UV detection wavelength of rhynchophylline and isorhynchophylline was set at 254 nm, UV detection wavelength of tetrahydropalmatine was set at 281 nm. RESULTS: The averagere recoveries of tetrahydropalmatine,rhynchophylline and isorhynchophylline were 98.47% ,99.04% and 98. 75% respectively; RSD were 0.95% ,2.6% and 1.6%, respectively. CONCLUSION: This method is simple, sensitive, accurate, and can be used for determining tetrahydropalmatine ,rhynchophylline and isorhynchophylline in Anshen Yangxue Oral Liquid.