Mechanism of macrophage apoptosis induced by Salmonella typhi / 中国人兽共患病学报
Chinese Journal of Zoonoses
; (12): 239-242, 2010.
Article
in Zh
| WPRIM
| ID: wpr-433303
Responsible library:
WPRO
ABSTRACT
To investigate the mechanism of macrophages apoptosis induced by stressed Salmonella typhi,macrophages were co-cultivate with inhibitors caspase 3,8,9 and anti-TNF-α antibody and then S.typhi was added to construct the infection model..The rate of macrophage apoptosis was assayed by flow cytometry and the contens of caspase 3.8 ,9 and anti-TNF-α antibody as well as NO were then determined respectively.It was found that the apoptotic rates of macrophages were significantly inhibited by caspase 3 and caspase 8 inhibitors and antibody against TNF-α respectively (P<0.01).A significantly enhanced generation of caspase 3 and caspase 8 activities during macrophage apoptosis induced by S.typhi correlated with the increased generation of TNF-αand NO (P<0.01).These results indicate that the inactive NO and TNF-α mediated inhibitors caspase 3 and caspase 8 participate the exogenous apoptotitic pathway of macrophages induced by S.typhi.
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Index:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Zoonoses
Year:
2010
Type:
Article