Determination of cholic acid and muscone in Jawei Xihuang Soft Capsule / 中成药
Chinese Traditional Patent Medicine
;
(12): 429-432, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-433333
ABSTRACT
AIM:
To develop a method for determining cholic acid by HPLC-ELSD and GC was applied to determing muscone;in Jawei Xihuang Soft Capsule(Calculus Bovis,Moschus,Venenum Bufonis,Olibanum,Myrrha).METHODS:
AC_(18) column(Kromasil C_(18),5 μm,4.6 mm×250 mm)was used as stationary phase,the mobile phase was methanol-0.01% glacial acetic acid(7321)at a flow rate of 1.0 mL/min.The parameters of ELSD were set as followsevaporation temperature was 40℃,carrier gas(N_2)pressure was 200 kPa.The GC system consisted of DB-1 capillary column(30 m×0.32 mm×0.25 μm)and FID as the detector.The programmed temperature-GC and internal standard method were employed to determine the content of muscone.RESULTS:
The linear ranges of cholic acid and muscone were in the range of 45.2 ng-904 ng and 0.05 mg/mL-0.5 mg/mL respectively.The average recoveries were 99.06% and 99.40% with RSD of 1.56% and 0.95% respectively.CONCLUSION:
The method is convenient and accurate,and it can be used for the quality evaluation of Jawei Xihuang Soft Capsule.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional Patent Medicine
Year:
2010
Type:
Article
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