Optimization of extraction of total flavonoids from Hypericum ascyron by Box-Behnken design / 中成药
Chinese Traditional Patent Medicine
;
(12): 389-392, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-433337
ABSTRACT
AIM:
To put forward three-level design(Box-Behnken design)for fitting response surfaces to optimize the extraction technology of total flavonoids from Hypericum ascyron L.METHODS:
Four factors,including temperature,reflux time,concentration of ethanol,ratio of solvent to raw material were used to examine the yield of total flavonoids.Prediction was carried out through comparing the observed and predicted values.RESULTS:
The results suggested that ethanol concentration and extraction temperature were two statistically significant factors.The optimum conditions of extraction process consisted of the ratio(mLg)of solvent to material(13.31),ethanol concentration(53.2%),extraction temperature(78.7℃)and extraction time(2.3h).Regression coefficient of binomial fitting complex model was as high as 0.984 6.Bias between observed and predicted values was-4.01%.CONCLUSION:
Box-Behnken design is success in optimizing the extaction in close agreement with the predicted values of the mathematic model.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Prognostic study
Language:
Chinese
Journal:
Chinese Traditional Patent Medicine
Year:
2010
Type:
Article
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