In vitro culture of human embryonic striatum-derived neural stem cells**★ / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
;
(53): 5048-5056, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-433691
ABSTRACT
BACKGROUND:
Neural stem cells are always derived from animals, and unsuitable for human transplantation treatment.OBJECTIVE:
To explore the in vitro culture methods of human embryonic striatum-derived neural stem cells, and to observe the biological characteristics.METHODS:
The human embryonic striatum were separated from the embryo at a gestational age of 8-16 weeks that received induction of labor with water bag, and then the embryonic striatum was in vitro cultured in the serum-free Dulbecco’s modified Eagle’s medium. The cells were passaged after neurospheres formation, and then the cells were induced to differentiation with the Dulbecco’s modified Eagle’s medium/F12 containing 10% fetal bovine serum. RESULTS ANDCONCLUSION:
The in vitro cultured human embryonic striatum-derived neural stem cells grew rapidly and could express nestin. Colony formation assay showed the cel clone formation rate was 6.0%-7.0%. 5-Bromodeoxyuridine incorporation assay showed the cel proliferation rate was 37.9%. Immunofluorescence staining showed that the cells after induction and differentiation could express Tuj-1, glial fibril ary acidic protein and nestin, but not express myelin basic protein. The results indicate that human embryonic striatum-derived neural stem cells cultured in the serum-free medium can maintain their biological characteristics and have self-renewal capacity, and the cells can differentiate into the neurons and astrocytes induced by the fetal bovine serum.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Tissue Engineering Research
Year:
2013
Type:
Article
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