A study on biological characteristics of normal adult human nasal septal chondrocytes cultured in vitro / 临床耳鼻咽喉头颈外科杂志

ABSTRACT

Objective:To study the characteristics of normal adult human nasal chondrocytes cultured in vit-ro. Method:The adult human nasal septal chondrocytes were cultured in vitro. The changes of cellular morpholo-gy from primary to subculture were investigated ;to observe the proliferation of chondrocytes by the growth curveand the survive rate of chondrocytes;and to determine the biologic synthesis of the chondrocytes by toluidineblue,alkaline phosphatase staining and type Ⅱ collagen immunohistochemistry reaction. The survive rate of thenasal septal chondrocytes was observed after being frozen (－196C). Result: The morphology of the primarychondrocytes maintained the spherical shape ,and some became polygonal shape after 4 days. Most of the chondro-cytes transformed into the shape of the fibroblast cell since the fifth generation. The number of the chondrocytes(2th generation)after being cultured in vitro for 5 days was approximately 4 fold of that of the plating. The phe-notype of the chondrocytes was maintained in the first 4 generation,the survive rate of the chondrocytes suscitat-ing from being frozen (－196C ) was 93 %. Conclusion:The normal adult nasal septal chondrocytes cultured inmonolayer in vitro maintained the specific chondrocytes phenotype in the first 4 generation,the growth was nor-mal ,and could survive in the condition of deep-frozen.