EVALUATION OF THE INVASIVE ABILITY OF TRASITIONAL-CELL BLADDER CARCINOMA BY THE MTT COLORIMETRIC ASSAY / 肿瘤

ABSTRACT

Objective　To evaluate the invasive ability of bladder transitional carcinoma cells(TCC),with a new method.Methods　MTT colorimetric assay combining with Boyden chamber system was used to measure the invasive ability of three bladder TCC lines EJ、T24、BIU-87. Invasive Index=experimental absorbance/control absorbance×100%.Tumor cells mortility growth curve and coloney forming test were measured for these 3 lines.Results　The Invasive Index of three cell lines were EJ 0.326%±0.046%，T24 0.569%±0.044% and BIU-871.256%±0.066%. The Motility Rate were EJ 0.508%±0.037%，T24 0.672%±0.039% and BIU-871.524%±0.043%.Conclusion　This method can evaluate the invasive ability of tumor cells accurately and it is sensitive,safe and rapid. It also can be done easily and has no radiation pollution.