INDUCTION OF APOPTOSIS IN HUMAN BLANDDER CANCER CELL HTB9 AND REGULATION OF APOPTOSIS RELATED GENE EXPRESSION BY WILD-TYPE p53 GENE / 肿瘤
Tumor
;
(12): 29-31, 2001.
Article
in Chinese
| WPRIM
| ID: wpr-433777
ABSTRACT
Objective To study the induction of apoptosis and the regulation of apoptosis related genes bcl-2, bax and ICE expression by wild-type p53 gene. Methods A p53 gene recombinant adenovirus vector, ad CMV p53, was transfected into human bladder cancer cell HTB9. The level of bax mRNA was quantified by reverse transcription-polymerase chain reaction (RT-PCR). The content of Bcl-2, Bax and ICE protein were detected by immunochemical staining. Agarose gel electrophoresis of DNA、 terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) method and flow cytometric technique were used for analysis of apoptosis. Results Introduction of wild-type p53 could increase the mRNA and protein level of bax, and could induce apoptosis in HTB9 cells with the apoptotic percentage of 50.4%. At the same time, the introduction also increased the protein level of ICE, but that of Bcl-2 decreased.Conclusion It's very possible that wild-type p53 gene induced apoptosis by regulating the expression of apoptosis-related genes including bax, bcl-2 and ICE.
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Tumor
Year:
2001
Type:
Article
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