EFFECTS AND CLINICAL SIGNIFICANCE OF PROGLUMIDE IN GASTRIN-STIMULATING CELL PROLIFERATION ON HUMAN COLON CELL LINE SW480 / 肿瘤

ABSTRACT

Objective　To investigate the role and clinical significance of proglumide (PGL) in gastrin-stimulating cell proliferation on human colon cell line SW480.Methods　Effects of proglumide on the viable cell count (A value), IP3 (CPM value), ［Ca2＋］i and PKC activity of human colon cell line SW480 were evaluated in vitro by MTT assay, 3H-myo-inositol incorporation, fluorescence measurements and γ-32P-ATP incorporation. Results　The results showed that A value of PGL＋PG group is lower than that of PG or control group (P＜0.01 in both groups); The concentration of IP3 or ［Ca2＋］i in PGL＋PG group is lower than that in PG group (P＜0.05). Conclusion　These results indicate effects of proglumide may be through the phosphoinositide signalling pathway on gastrin-stimulating cell proliferation in human colon cell line SW480. It will provide an experimental evidence for antisingnal transduction therapy for patients with colon cancer.