Determination of Pd-Ⅰa and Pd-Ⅱ in Peucedanum praeruptorum by GC / 中国药学杂志

ABSTRACT

OBJECTIVE　To establish a GC method for the determination of the contents of Pd-Ⅰa and Pd-Ⅱ in roots of Peucedanum Praeruptorum.METHODS　The determination was performed on a silica capillary SE-30(0.25 μm 0.22 mm×25 m)column by FID detector;with n-C25H52 as the internal standard.RESULTS　The standard curves of Pd-Ⅰa and Pd-Ⅱ were linear over the range from 0.175 μg to 1.919 μg(r=0.9995) and 0.086 μg to 0.950 μg (r=0.9996),respectively.The average recoveries were (98.28±2.07)% for Pd-Ⅰa and (99.58±2.44)% for Pd-Ⅱ.Compared with HPLC,there was no significant difference in the results between GC and HPLC.CONCLUSION　The method appeared to be simple,sensitive,rapid and accurate and can be used for the quality control of Chinese medicine Qianhu.