Display of human decay-accelerating factor on the surface of yeast / 免疫学杂志

ABSTRACT

Objective　To display efficient folding hDAF on the surface of yeast. Methods　The hDAF open reading frame was cloned by PCR from DAF- pBluescript M13-（Amp＋）plasmid， then subcloned into the yeast surface displayed vector pYD1.The recombinant vector was transformed into yeast cells EBY100.Flow cytometric analysis was carried out to evaluate direct binding of anti-DAF mAbs onto the surface of yeast cells displaying DAF. Results　Three mAbs against DAF different epitopes could bind onto DAF displayed on the surface of yeast.Conclusion　Efficient folding DAF can be displayed on the surface of yeast potentially leading to the development of novel applications involving DAF yeast display.