Recombination and expression of a component of urease B subunit transmembrane protein of helicobacter pylori / 免疫学杂志
Immunological Journal
;
(12): 94-96, 2001.
Article
in Chinese
| WPRIM
| ID: wpr-433891
ABSTRACT
Objective To recombine and express a component of urease B subunit transmembrane protein of helicobacter pylori. Methods A 732 bp gene fragment of urease B subunit of helicobacter pylori was cloned into pET11C and transformed into BL21(DE3)E.coli. The positive clone was induced with IPTG. The expression of target protein was analysed by SDS-PAGE and Western blot. Results It is successful to construct the recombinant plasmid pET-UreB0.7 containing urease B subunit 0.7 kb gene fragment. A protein (MW≈28 000 u) with immunoreactivity, was expressed by 19.8% in BL21(DE3)E.coli induced with IPTG. Conclusions The recombinant component of urease B subunit transmembrane protein may play a role in the research of its biological function and might be used as the vaccine against helicobacter pylori.
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Immunological Journal
Year:
2001
Type:
Article
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