Specific anti-toxocara IgG and IgE responses in the patients with ocular toxocariasis / 中国人兽共患病学报

ABSTRACT

To investigate the prevalence of specific anti-Toxocara IgG and IgE antibodies in ocular toxocariasis by means of ELISA and Western blotting, serum samples of 105 cases with uveitis of unidentified etiology were randomly selected from our stocked sera, which were referred to the Section of of Environmental Parasitology of Tokyo Medical and Dental University for detection of the anti-Toxocara antibody. By using ELISA, 82 of them (78.1%) were negative for both IgG and IgE antibodies, 12 (11.4%) were positive only for IgG, three (2.9%) were positive only for IgE, and eight (7.6%) were positive for IgG and IgE. Among the positive samples, as demonstrated by western bloting the IgG reacting bands were found to distribute in the whole range of molecular weights (97.2-14.3kDa)of excretory-secretory products of T. canis larvae. On the other hand,IgE antigenic molecules were concentrated on a relatively narrow range from 45kDa to 29kDa. In this study, we clearly demonstrated that some of the patients with uveitis showed specific anti-Toxocara IgE antibody but not IgG antibody in serum sample, suggesting that the demonstration of specific IgE antibody should be considered for the precise diagnosis of ocular toxocariasis. Further studies are needed to clarify the diagnosis significance of specific IgE antibody in ocular toxocariasis.