Studies on Water-soluble substances and Determination of Danshensu and protocatechualdehyde of Herba Salvia chinensis / 中国实验方剂学杂志

ABSTRACT

Objective: To study and determine the Water-soluble substances of Salvia chinensis. Methods: The constituents of the n-Butanol-soluble portion of the water extractive were isolated and purified by means of Sephadex LH20 column chromatographic methods. HPLC was used to determine the contents. Results: Two compounds were isolated and identified as danshensu and rosmarinic acid. The linear range of danshensu was 1.48～7.40 μg. The average recovery was 102.8% and RSD was 1.04% ; The linear range of protocatechualdehyde was 0.05～0.26μg. The average recovery was 101.5% and RSD was 0.72%. Conclusion: The method can provide useful references to quality control of Herba Salvia chinensis. Danshensu was firstly isolated from Herba Salvia chinensis.