Naltrexone influences protein kinase C epsilon and integrin alpha7 activity in SH-SY5Y neuroblastoma cells
Experimental & Molecular Medicine
;
: 100-106, 2006.
Article
in English
| WPRIM
| ID: wpr-43449
ABSTRACT
Alcohol influences the neuroadaptation of brain cells where receptors and enzymes like protein kinase C (PKC) exist. Naltrexone acts on opioid receptors. However, other mechanisms of action remain unknown. We prepared SH-SY5Y neuroblastoma cells, and fed them with 150 mM ethanol for 72 h followed by treatment with naltrexone for 24 h. We performed microarray analysis and reverse transcriptase-polymerase chain reaction. Our results showed that PKC epsilon increased 1.90 times and showed an overall decreasing pattern as time increased. Phosphorylated ERK also increased 2.0 times according to the change of PKC epsilon. Integrin alpha7 increased 2.32 times and showed an increasing pattern as time increased. In conclusion, naltrexone influences PKC epsilon neuronal signaling system and endothelial adhesion molecule integrin alpha7 in addition to the well-known opioid system.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Time Factors
/
Comparative Study
/
Antigens, CD
/
DNA, Complementary
/
Reverse Transcriptase Polymerase Chain Reaction
/
Oligonucleotide Array Sequence Analysis
/
Integrin alpha Chains
/
Cell Line, Tumor
/
Protein Kinase C-epsilon
/
Naltrexone
Limits:
Humans
Language:
English
Journal:
Experimental & Molecular Medicine
Year:
2006
Type:
Article
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