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Application of COI-based DNA Barcoding for Identifying Animal Medical Materials in the Chinese Pharmacopoeia / 世界科学技术-中医药现代化
World Science and Technology-Modernization of Traditional Chinese Medicine ; (12): 371-380, 2013.
Article in Chinese | WPRIM | ID: wpr-435219
ABSTRACT
Medicinal animals are important part of Traditional Chinese medicine resources in China. Cytochrome c oxidase subunit I (COI) was selected as the standard DNA barcoding sequence for animal medical materials. In this study, the 51 animal species from 45 animal medical materials in the Chinese Pharmacopoeia were selected and the intra-specific variation and the inter-specific divergence, the barcoding gap, the identification efficiency of their COI sequences were analyzed. The results showed that the inter-specific divergence is higher than intra-specific distance. The barcoding gap existed between inter-specific sequence divergence and intra-specific dis-tance. The identification efficiencies were 100% both at the genus and species level except the Arthropoda. The cluster dendrogram exhibited that different species distinguished from others. Therefore, COI sequence as a bar-code is suitable to identify the species of animal medical materials in Chinese Pharmacopoeia.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: World Science and Technology-Modernization of Traditional Chinese Medicine Year: 2013 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: World Science and Technology-Modernization of Traditional Chinese Medicine Year: 2013 Type: Article