Effect of glucocorticoids on expression of aquaporin-1 in rats with acute lung injury / 中国小儿急救医学
Chinese Pediatric Emergency Medicine
;
(12): 265-267, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-435368
ABSTRACT
Objective To explore the mechanism of glucocorticoids in the treatment of acute lung injury(ALI) by studying the expression changes of aquaporin-1 (AQP-1) in the lung.Methods SpragueDawley rats were randomly divided into 3 groups,which were ALI group injected by lipopolysaccharide (LPS group),therapy group treated by methylprednisolone (Mp) after LPS injection (Mp group) and control group injected by normal saline.PaO2 and wet/dry mass ratio were assayed at 6 hours after the injection.Changes of AQP-1 expression in the lungs were analyzed by immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR),and pathological examination was performed in each group.Results RT-PCR results showed that the expression of AQP-1 in LPS group was lower than that in control group (0.28 ± 0.05 vs 0.50 ± 0.24,P < 0.05),while the expression of AQP-1 in Mp group was higher than that in LPS group (0.47 ±0.16 vs 0.28 ±0.05,P <0.05).The immunohistochemistry results were consistent to the RT-PCR results,meanwhile glucocorticoids therapy could improve the blood oxygen level (P < 0.01),reduce the wet/dry mass ratio (P < 0.05) and relieve the lung injury.Conclusion Glucocorticoids may have beneficial effects on ALI by affecting the expression of AQP-1 in the lungs of rats.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Pediatric Emergency Medicine
Year:
2013
Type:
Article
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