Genotype identification of the 18S rDNA in Acanthamoeba species isolated from tap water in Yanji city of Jilin province / 中国人兽共患病学报

ABSTRACT

To identify the genotype of the 18S rDNA in Acanthamoeba species isolated from tap water inYanji city of Jilin province, the full genomic DNA sequence of the CJY/W1 strain of Acanthaqmoeba was amplified by PCR and then the amplified products were sequenced. The full-length of DNA sequence was analyzed with Clustal and Genedoe softwares. It was found that the full-length of gene of the Acanthamoeba CJY/W1 strain isolated from tap water was 2252 bps. The 18S rDNA genotype of this strain appeared to be near to the T1 type. But the percentage of the genetic difference between T1 type was 8%. According to the classification criteria of 18S rDNA, the genotype of the CJY/W1 strain might be a new one.